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KMID : 0624620130460090448
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BMB Reports
2013 Volume.46 No. 9 p.448 ~ p.453
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CD83 expression induced by CpG-DNA stimulation in a macrophage cell line RAW 264.7
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Park Min-Chul
Kim Dong-Bum
Lee Young-Hee
Kwon Hyung-Joo
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Abstract
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CpG-DNA has various immunomodulatory effects in dendritic cells, B cells, and macrophages. While induction of cytokines by CpG-DNA has been well documented in macrophages, the expression of costimulatory molecules in CpG-DNA treated macrophages has not yet been defined. Therefore, we investigated the effects of CpG-DNA on the expression of costimulatory molecules in RAW 264.7 cells. The surface expression of CD80 was slightly increased and CD83 expression was significantly increased in response to CpG-DNA. However, the expression of CD86 and MHC class II was not changed. As expression of CD83 mRNA was also increased by CpG-DNA, CD83 expression is regulated at a transcriptional level. To understand the contribution of signaling pathways to CD83 induction, we used pathway specific inhibitors. The NF-κB inhibitor significantly reduced surface expression of CD83 as well as phagocytic activity of RAW 264.7 cells. Therefore, CD83 expression may contribute to the immunostimulatory effects of CpG-DNA in macrophage cells.
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KEYWORD
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CD83, Costimulatory molecule, CpG-DNA, Innate immunity, Macrophage
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